Find Nuclei 1,061,277 Cell Nuclei From 100 Experiments
Every nucleus imaged across 100 experiments, cropped and stitched into one 263,936 × 263,680 pixel OME-Zarr with a 10-level pyramid, about 70 GB at full resolution. Dataset by Russell Barkley, hosted on the Open Storage Network and read directly from there. Zoom out for the whole collection, zoom in for chromatin texture. Related preprint: Barkley RJR, Brodrick AJ, Parker JSL (2026). Reimag(in)ing representative images. Elemental Microscopy. doi:10.69761/ggmj4852
Reovirus infection, thirty timepoints
The time-lapse from the same preprint: mNeonGreen and mCherry, two channels over thirty frames. The figure plays on its own once it is on screen and pauses while scrolled away. Every frame is shown complete, so on a slow link the tempo drops rather than the frame tearing. Hover for the play button and the time slider.
Data: Barkley RJR (2026). Nuclei mosaic. doi:10.5281/zenodo.18776682, CC BY 4.0. Streamed as OME-Zarr from the Open Storage Network (Kirkpatrick et al., 2021, doi:10.6075/J0RR1ZCR).
Methods: Barkley RJR (2026). Fluorescent Protein Tag Confounds in Reovirus Biology, pp. 75–79. PhD dissertation, Cornell University. ProQuest, ISBN 9798190931174.
Acknowledgement: This work used the Open Storage Network through allocation BIO250450 from the Advanced Cyberinfrastructure Coordination Ecosystem: Services & Support (ACCESS) program, which is supported by U.S. National Science Foundation grants #2138259, #2138286, #2138307, #2137603, and #2138296. Boerner et al. (2023), doi:10.1145/3569951.3597559.
Video: Barkley RJR, Brodrick AJ, Parker JSL (2026). Live imaging from Reimag(in)ing representative images, Elemental Microscopy, doi:10.69761/ggmj4852, CC BY 4.0. Streamed as OME-Zarr from the Open Storage Network.
Viewer: Find Nuclei (2026). Find Nuclei Viewer, release 2026-09-19. https://find-nuclei.github.io · CITATION.cff
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