Sample Data and Credits

Every screenshot in these guides uses public data. Nothing here is customer material.

If you reuse one of these images, cite the source rather than this site.

Whole-slide H&E, CMU-1

Used in the Annotations and Annotation Templates guides, and for the brightfield display states in Channels.

A freely distributed test slide from the OpenSlide project, converted to OME-Zarr with bioformats2raw. Widely used for testing whole-slide readers.

High-content screen, BBBC013

Used in Analytics & Phenotyping, parts of Labels, and for the fluorescence display states in Channels.

Human U2OS cells, cytoplasm-to-nucleus translocation, from the Broad Bioimage Benchmark Collection.

Ljosa V, Sokolnicki KL, Carpenter AE (2012). Annotated high-throughput microscopy image sets for validation. Nature Methods 9(7):637. doi:10.1038/nmeth.2083

The segmentation masks and measurements shown alongside it were produced by running StarDist and Cellpose over that image set. They are our output, not part of the original collection.

Fluorescence nuclei, 6001240

Used in Labels.

Image 6001240 from the Image Data Resource, study idr0062, dataset idr0062-blin-nuclearsegmentation/experimentA. Served from IDR's public bucket. The viewer's own live examples read it directly from IDR rather than re-hosting it.

Blin G, Sadurska D, Migueles RP, Chen N, Watson JA, Lowell S (2019). Nessys: A new set of tools for the automated detection of nuclei within intact tissues and dense 3D cultures. PLOS Biology 17(8):e3000388. doi:10.1371/journal.pbio.3000388

Published under CC BY 4.0. Copyright Blin et al.

Plate, 76-45

Used in HCS Plates and for the channel controls in Channels.

Plate 76-45 from the Image Data Resource, study idr0010, screen idr0010-doil-dnadamage/screenA. An RNAi screen for genes whose knockdown changes how 53BP1 accumulates at DNA double-strand breaks. The copy we read was converted to OME-Zarr 0.5 for the OME 2024 NGFF Challenge, a community effort to publish a large body of public imaging in the current NGFF version.

Doil C, Mailand N, Bekker-Jensen S, Menard P, Larsen DH, Pepperkok R, Ellenberg J, Panier S, Durocher D, Bartek J, Lukas J, Lukas C (2009). RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Cell 136(3):435-446. doi:10.1016/j.cell.2008.12.041

Published under CC BY 4.0. Copyright Doil et al.

Synthetic RGB

Used for the RGB control in Channels.

Generated by us as a test fixture. Not real microscopy, and not something to cite.